LOCUS       40924_35703        6320 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pPVLUC441 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6320)
  AUTHORS   Hashinaka K.
  TITLE     Synthetic Autonomous Vectors Based on Palindromic Sequences of 
            Parvovirus B19
  JOURNAL   Published Only in Database (2001)
REFERENCE   2  (bases 1 to 6320)
  AUTHORS   Hashinaka K.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2000) Kazuya Hashinaka, Miyazaki Medical College, 
            Department of Biochemistry; 5200 Kihara, Kiyotake, Miyazaki 
            889-1692, Japan (E-mail:hasinaka@post1.miyazaki-med.ac.jp, 
            Tel:81-985-85-0985, Fax:81-985-85-2401)
REFERENCE   3  (bases 1 to 6320)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6320)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Published 
            Only in Database (2001)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (21-FEB-2000) Kazuya Hashinaka, Miyazaki Medical College, Department
            of Biochemistry"; volume: " 5200 Kihara, Kiyotake, Miyazaki 
            889-1692, Japan (E-mail:hasinaka@post1.miyazaki-med.ac.jp, 
            Tel:81-985-85-0985, Fax"; pages: "81-985-85-2401"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6320
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          6..479
                     /mol_type="other DNA"
                     /label=synonym:Parvovirus B19
                     /note="synonym:Parvovirus B19"
                     /db_xref="taxon:10798"
                     /organism="Human parvovirus B19"
     source          2937..3420
                     /mol_type="other DNA"
                     /label=synonym:Parvovirus B19
                     /note="synonym:Parvovirus B19"
                     /db_xref="taxon:10798"
                     /organism="Human parvovirus B19"
     repeat_region   9..391
     promoter        522..720
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     regulatory      750..759
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1347..2414
                     /codon_start=1
                     /gene="luc"
                     /product="luciferase"
                     /label=luc
                     /protein_id="BAB32737.1"
                     /translation="MNSSGSTGLPKGVALPHRTACVRFSHARDPIFGNQIIPDTAILSV
                     VPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYKIQSALLVPTLFSFFAKST
                     LIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGYGLTETTSAILITPEGDDK
                     PGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMSGYVNNPEATNALIDKDGW
                     LHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESILLQHPNIFDAGVAGLPDDD
                     AGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVVFVDEVPKGLTGKLDARKI
                     REILIKAKKGGKIAV"
     gene            1347..2414
                     /gene="luc"
                     /label=luc
     polyA_signal    complement(2455..2576)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     repeat_region   3029..3411
     promoter        complement(3429..3447)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3454..3470)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      3611..4066
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4147..4251
                     /label=AmpR promoter
     CDS             4252..5109
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      5283..5871
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    6159..6180
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        6195..6225
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6233..6249
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6257..6273
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        6291..6309
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 atgcatggcc aaatcagatg ccgccggtcg ccgccggtag gcgggacttc cggtacaaga
       61 tggcggacaa ttacgtcatt tcctgtgacg tcatttcctg tgacgtcact tccggtgggc
      121 gggacttccg gaattagggt tggctctggg ccagcgcttg gggttgcctt gacactaaga
      181 caagcggcgc gccgcttgat cttagtggca cgtcaacccc aagcaagctg gcccagagcc
      241 aaccctaatt ccggaagtcc cgcccaccgg aagtgacgtc acaggaaatg acgtcacagg
      301 aaatgacgta attgtccgcc atcttgtacc ggaagtcccg cctaccggcg gcgaccggcg
      361 gcatctgatt tggtgtcttc ttttaaattt tagcgggctt ttttcccgcc ttatgcaaat
      421 gggcagccat tttaagtgtt ttactataat tttattggtt agttttgtaa cgaaggtacc
      481 gagctcttac gcgtgctagc ccgggctcga gatctgcgat ctgcatctca attagtcagc
      541 aaccatagtc ccgcccctaa ctccgcccat cccgacccct aactccgccc agttccgccc
      601 attctccgcc ccatcgctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
      661 cctctgagct attccaagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
      721 aagcttggca ttccggtact gttggtaaag ccaccatgga agacgccaaa aacataaaag
      781 aaaggcccgg cgccattcta tccgctggaa gatggaaccg ctggagagca actgcataag
      841 gctatgaaga gatacgccct ggttcctgga acaattgact tttacagatg cacatatcga
      901 ggtggacatc acttacgctg agtacttcga aatgtccgtt cggttggcag aagctatgaa
      961 acgatatggg ctgaatacaa aatcacagaa tcgtcgtatg cagtgaaaac tctcttcaat
     1021 tctttatgcc ggtgttgggc gcgttattta tcggagttgc agttgcgccc gcgaacgaca
     1081 atttataatg aacgtgaatt gctcaacagt atgggcattt cgcagcctac cgtggtgttc
     1141 gtttccaaaa aggggttgca aaaaattttg aacgtgcaaa aaaaagctcc caatcatcca
     1201 aaaaattatt atcatggatt ctaaaacgga ttaccaggga tttcagtcga tgtacacgtt
     1261 cgtcacatct catctacctc cacggtttta atgaatacga ttttgtgcca gagtccttcg
     1321 atagggacaa gacaattgca ctgatcatga actcctctgg atctactggt ctgcctaaag
     1381 gtgtcgctct gcctcataga actgcctgcg tgagattctc gcatgccaga gatcctattt
     1441 ttggcaatca aatcattccg gatactgcga ttttaagtgt tgttccattc catcacggtt
     1501 ttggaatgtt tactacactc ggatatttga tatgtggatt tcgagtcgtc ttaatgtata
     1561 gatttgaaga agagctgttt ctgaggagcc ttcaggatta caagattcaa agtgcgctgc
     1621 tggtgccaac cctattctcc ttcttcgcca aaagcactct gattgacaaa tacgatttat
     1681 ctaatttaca cgaaattgct tctggtggcg ctcccctctc taaggaagtc ggggaagcgg
     1741 ttgccaagag gttccatctg ccaggtatca ggcaaggata tgggctcact gagactacat
     1801 cagctattct gattacaccc gagggggatg ataaaccggg cgcggtcggt aaagttgttc
     1861 cattttttga agcgaaggtt gtggatctgg ataccgggaa aacgctgggc gttaatcaaa
     1921 gaggcgaact gtgtgtgaga ggtcctatga ttatgtccgg ttatgtaaac aatccggaag
     1981 cgaccaacgc cttgattgac aaggatggat ggctacattc tggagacata gcttactggg
     2041 acgaagacga acacttcttc atcgttgacc gcctgaagtc tctgattaag tacaaaggct
     2101 atcaggtggc tcccgctgaa ttggaatcca tcttgctcca acaccccaac atcttcgacg
     2161 caggtgtcgc aggtcttccc gacgatgacg ccggtgaact tcccgccgcc gttgttgttt
     2221 tggagcacgg aaagacgatg acggaaaaag agatcgtgga ttacgtcgcc agtcaagtaa
     2281 caaccgcgaa aaagttgcgc ggaggagttg tgtttgtgga cgaagtaccg aaaggtctta
     2341 ccggaaaact cgacgcaaga aaaatcagag agatcctcat aaaggccaag aagggcggaa
     2401 agatcgccgt gtaattctag agtcggggcg gccggccgct tcgagcagac atgataagat
     2461 acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg
     2521 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca
     2581 acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa
     2641 gcaagtaaaa cctctacaaa tgtggtaaaa tcgataagga tctgaacgat ggagcggaga
     2701 atgggcggaa ctgggcggag ttaggggcgg gatgggcgga gttaggggcg ggactatggt
     2761 tgctgactaa ttgagatgca tgctttgcat acttctgcct gctggggagc ctggggactt
     2821 tccacacctg gttgctgact aattgagatg catgctttgc atacttctgc ctgctgggga
     2881 gcctggggac tttccacacc ctaactgaca cacattccac agcggatccg tcgagtctag
     2941 tgggcagatc ttaagattgt gtaatatgta tcaaaattta gaaaaataaa catttattgt
     3001 ggttaaaaaa ttatgttgtt gcgctttaaa aatttaaaag aagacaccaa atcagatgcc
     3061 gccggtcgcc gccggtaggc gggacttccg gtacaagatg gcggacaatt acgtcatttc
     3121 ctgtgacgtc atttcctgtg acgtcacttc cggtgggcgg gacttccgga attagggttg
     3181 gctctgggcc agcttgcttg gggttgacgt gccactaaga tcaagcggcg cgccgcttgt
     3241 cttagtgtca aggcaacccc aagcgctggc ccagagccaa ccctaattcc ggaagtcccg
     3301 cccaccggaa gtgacgtcac aggaaatgac gtcacaggaa atgacgtaat tgtccgccat
     3361 cttgtaccgg aagtcccgcc taccggcggc gaccggcggc atctgatttg gccacgggcc
     3421 caattcgccc tatagtgagt cgtattacaa ttcactggcc gtcgttttac aacgtcgtga
     3481 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
     3541 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
     3601 tggcgaatgg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     3661 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     3721 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     3781 ttccgattta gagctttacg gcacctcgac cgcaaaaaac ttgatttggg tgatggttca
     3841 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     3901 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     3961 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     4021 caaatattta acgcgaattt taacaaaata ttaacgttta caatttcgcc tgatgcggta
     4081 ttttctcctt acgcatctgt gcggtatttc acaccgcata caggtggcac ttttcgggga
     4141 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     4201 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     4261 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     4321 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     4381 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     4441 tttccaatga tgagcacttt taaagttctg ctatgtcata cactattatc ccgtattgac
     4501 gccgggcaag agcaactcgg tcgccgggcg cggtattctc agaatgactt ggttgagtac
     4561 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     4621 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     4681 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     4741 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     4801 atgccaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     4861 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     4921 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     4981 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     5041 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     5101 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     5161 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     5221 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     5281 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     5341 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     5401 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac
     5461 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     5521 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     5581 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     5641 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     5701 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     5761 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     5821 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatcgaa aaacgccagc
     5881 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     5941 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     6001 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     6061 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
     6121 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
     6181 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
     6241 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagct atttaggtga
     6301 cactatagaa tactcaagct
//
